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collagenase type ii iv  (MedChemExpress)


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    Structured Review

    MedChemExpress collagenase type ii iv
    Collagenase Type Ii Iv, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/collagenase+iv/Collagenase/10__1161_slash_jaha__125__047180-98-60-63
    Average 95 stars, based on 62 article reviews
    collagenase type ii iv - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Single Cell:

    Article Title: Deep tumor access via tumor-specific mannose unmasking: Smart heptamannosylated β-cyclodextrin nanoassembly.
    Article Snippet: Tumor-resident immune cells, such as tumor-associated macrophages (TAMs), possess inherent tumorpenetrating capabilities and hold significant potential for actively transporting extravasated therapeutics through dense tumor stroma to eliminate deep-seated tumor cells.. Inspired by this mechanism, we engineered TAM-hitchhiking nanocarriers ("nano-hitchhikers") for deep-tumor drug delivery.. The nano-hitchhikers comprise heptamannosylated β-cyclodextrin loaded with doxorubicin (Dox), crosslinked via phenylboronic acidterminated anti-PD-L1 peptides with a diameter of ~100 nm.

    Article Title: Cucurbitacin B mitigates Staphylococcus aureus pathogenicity and reprograms macrophage responses to restore host defense
    Article Snippet: The healing kinetics were plotted via GraphPad Prism to generate wound healing curves. .. For immunological analysis, wound tissues were harvested and digested with a cocktail containing 1 mg/mL collagenase IV (MedChemExpress), 1 mg/mL collagenase I (MedChemExpress), and 100 μg/mL DNase I (Yeasen Biotechnology (Shanghai) Co., Ltd., Shanghai, China) at 37 °C for 1 h. Single-cell suspensions were obtained by filtering through a 70-μm mesh and treated with red blood cells (RBCs) lysis buffer (Biosharp, Beijing, China). .. The cells were then stained with fluorochrome-conjugated antibodies against Ly6G, CD11b, CD86, and CD206 to evaluate neutrophil infiltration and macrophage polarization (Multi Sciences).

    Suspension:

    Article Title: Deep tumor access via tumor-specific mannose unmasking: Smart heptamannosylated β-cyclodextrin nanoassembly.
    Article Snippet: Tumor-resident immune cells, such as tumor-associated macrophages (TAMs), possess inherent tumorpenetrating capabilities and hold significant potential for actively transporting extravasated therapeutics through dense tumor stroma to eliminate deep-seated tumor cells.. Inspired by this mechanism, we engineered TAM-hitchhiking nanocarriers ("nano-hitchhikers") for deep-tumor drug delivery.. The nano-hitchhikers comprise heptamannosylated β-cyclodextrin loaded with doxorubicin (Dox), crosslinked via phenylboronic acidterminated anti-PD-L1 peptides with a diameter of ~100 nm.

    Lysis:

    Article Title: Cucurbitacin B mitigates Staphylococcus aureus pathogenicity and reprograms macrophage responses to restore host defense
    Article Snippet: The healing kinetics were plotted via GraphPad Prism to generate wound healing curves. .. For immunological analysis, wound tissues were harvested and digested with a cocktail containing 1 mg/mL collagenase IV (MedChemExpress), 1 mg/mL collagenase I (MedChemExpress), and 100 μg/mL DNase I (Yeasen Biotechnology (Shanghai) Co., Ltd., Shanghai, China) at 37 °C for 1 h. Single-cell suspensions were obtained by filtering through a 70-μm mesh and treated with red blood cells (RBCs) lysis buffer (Biosharp, Beijing, China). .. The cells were then stained with fluorochrome-conjugated antibodies against Ly6G, CD11b, CD86, and CD206 to evaluate neutrophil infiltration and macrophage polarization (Multi Sciences).

    Gradient Centrifugation:

    Article Title: Mannosylated graphene oxide nanotherapeutics co-delivering docetaxel and a STING agonist reprogram myeloid cells and potentiate antitumor immunity
    Article Snippet: .. Tumor tissues were digested with collagenase IV, hyaluronidase and DNase I (MedChemExpress, USA) at 37 °C for 1 h. After digestion, intratumoral single cells were enriched by Percoll density gradient centrifugation (Solarbio, Beijing, China). ..

    Gentle:

    Article Title: ROS-Triggered Self-Aggregation of a β-Elemene Olefin-Rich Nanoemulsion for Mitochondrial-Targeted Metabolic Reprogramming and Colitis Inflammation Alleviation
    Article Snippet: .. Tissue digestion was performed for 60 min at 37 °C with gentle shaking in RPMI-1640 complete medium containing 2 mg/mL collagenase IV (MCE), 2.5 mg/mL Liberase (Roche), and 0.1 mg/mL DNase I (Sigma-Aldrich). ..

    Incubation:

    Article Title: Single-cell chromatin accessibility landscape of cardiac non-myocytes identifies tissue repair program during heart regeneration.
    Article Snippet: .. Tissue dissociation was performed by incubation in a solution of collagenase IV (400 U mL−1) (40510ES76, Yeasen), dispase (1.2 U mL−1) (HY-131577, MCE), and DNase I (32 U mL−1) (LS002138, Worthington) in PBS supplemented with Mg2+ and Ca2+. ..

    Article Title: Single-cell chromatin accessibility landscape of cardiac non-myocytes identifies tissue repair program during heart regeneration
    Article Snippet: .. Tissue dissociation was performed by incubation in a solution of collagenase IV (400 U mL−1) (40510ES76, Yeasen), dispase (1.2 U mL−1) (HY-131577, MCE), and DNase I (32 U mL−1) ( LS002138 , Worthington) in PBS supplemented with Mg2+ and Ca2+. ..

    Centrifugation:

    Article Title: Mannosylated graphene oxide nanotherapeutics co-delivering docetaxel and a STING agonist reprogram myeloid cells and potentiate antitumor immunity
    Article Snippet: .. 326 Tumor tissues were digested with collagenase IV, hyaluronidase and DNase I (MedChemExpress, 327 USA) at 37 °C for 1 h. After digestion, intratumoral single cells were enriched by Percoll density gradient 328 centrifugation (Solarbio life sciences &technologyco., Ltd., Beijing, China). ..



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    Image Search Results


    Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following collagenase-IV treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.

    Journal: STAR Protocols

    Article Title: Protocol for isolating viable human central nervous system T cells

    doi: 10.1016/j.xpro.2026.104464

    Figure Lengend Snippet: Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following collagenase-IV treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.

    Article Snippet: Collagenase IV , Worthington , Cat# LS004189.

    Techniques: RNA Sequencing, Gene Expression, Expressing